Margulis M S, Kvitsinskaia E A, Sorokin Iu A
Vestn Khir Im I I Grek. 1980 Apr;124(4):48-52.
The dynamics of changes of the general, "non-sedimenting" activity of organelle-specific enzymes as well as their activity in perfusate during hypothermal conservation of the liver were studied in 77 experiments by the method of continuous and discontinuous perfusion, without perfusion and during the period of normal thermally artificial blood circulation after 3 hours of the organ conservation. The complex of enzyme tests including the assessment of "non-sedimenting activity" of mitochondrial glutamatdehydrogenase and lysosomal acid desoxiribonuclease during the conservation period was found to be an adequate criterion of the liver conservation quality. After the recovery of the artificial blood flow in the conservated organ the conservation quality is also assessed by their activity in the perfusate, in addition to determination of urokinase activity in the perfusate.
通过连续和间断灌注、无灌注以及在器官保存3小时后的正常热人工血液循环期间等方式，在77个实验中研究了肝低温保存期间细胞器特异性酶的总体“非沉降”活性变化动态及其在灌注液中的活性。包括评估保存期间线粒体谷氨酸脱氢酶和溶酶体酸性脱氧核糖核酸酶的“非沉降活性”在内的酶测试组合，被发现是肝脏保存质量的一个充分标准。在保存器官中恢复人工血流后，除了测定灌注液中的尿激酶活性外，还通过它们在灌注液中的活性来评估保存质量。
