Stoltzner G H, Dorsey B A
Am J Clin Nutr. 1980 Jun;33(6):1264-71. doi: 10.1093/ajcn/33.6.1264.
Effects of chronic dietary protein restriction on immunological response were studied in BALB/c male mice. Three diets were used: 4, 8, 24% protein (casein) which 1000 mice were allowed to eat ad libitum. Restricted mice were not malnourished. Mice subjected to 4% protein diet had life expectancy that was marginally significantly prolonged when compared with "control" mice eating 24% protein diet. In vivo primary antibody responses to sheep erythrocytes, assessed by plaque formation and serum hemagglutination, were lower in 4% protein fed mice than in those eating 24% protein diets. Primary antibody responses fell with age in all diet groups. In vitro splenic lymphocytic blastogenesis, measured by 3H-thymidine uptake to concanavalin A, phytohemagglutinin, lipopolysaccharide, and allogenic lymphocytes, was not significantly influenced by diet. Thymus weight was not importantly influenced by diet. Spleens from mice eating 4% protein diet and immunized with sheep erythrocytes were significantly smaller than those from control animals. Our findings are consistent with several other reports, and indicate that this type of diet restriction does not delay onset of aging patterns found in the immune system.
在BALB/c雄性小鼠中研究了长期饮食蛋白质限制对免疫反应的影响。使用了三种饮食：蛋白质含量分别为4%、8%、24%（酪蛋白）的饮食，让1000只小鼠随意进食。限制饮食的小鼠没有营养不良。与食用24%蛋白质饮食的“对照”小鼠相比，食用4%蛋白质饮食的小鼠预期寿命略有显著延长。通过空斑形成和血清血凝测定，食用4%蛋白质饮食的小鼠对绵羊红细胞的体内初次抗体反应低于食用24%蛋白质饮食的小鼠。所有饮食组的初次抗体反应均随年龄下降。通过3H-胸腺嘧啶核苷摄取法测定对刀豆蛋白A、植物血凝素、脂多糖和同种异体淋巴细胞的体外脾淋巴细胞增殖，饮食对其没有显著影响。胸腺重量受饮食的影响不大。食用4%蛋白质饮食并用绵羊红细胞免疫的小鼠的脾脏明显小于对照动物的脾脏。我们的研究结果与其他几份报告一致，表明这种饮食限制不会延迟免疫系统中发现的衰老模式的出现。
