Bounous G, Létourneau L, Kongshavn P A
J Nutr. 1983 Jul;113(7):1415-21. doi: 10.1093/jn/113.7.1415.
The effect of graded amounts of dietary lactalbumin (L), casein (C), soy (S), wheat (W) protein and Purina rodent chow (stock diet) on the immune responsiveness of C3H/HeN mice has been investigated by measuring the specific humoral immune response to sheep red blood cells (SRBC), and horse red blood cells (HRBC) as well as the nonspecific splenic cell responsiveness to phytohemagglutinin (PHA) and concanavalin A (Con A) after stimulation with Mycobacterium bovis, strain BCG. The nutritional efficiency of these diets was normal and similar. The immune response of mice fed the L diets, was found to be almost five times higher than that of mice fed the corresponding C diets. The humoral immune response of mice fed C, S, and W diets was substantially lower than that of mice fed stock diet, whereas that of mice fed L diet was higher. The above-described immune effect of all tested proteins was obtained at 20 g/100 g concentration with no further increments with 30- and 40 g/100 g protein in the diet. Mitogen responsiveness to PHA and Con A in L diet-fed mice was only slightly higher than that of C diet-fed mice. Little difference in immune responses was noted among mice fed C, S or W protein diets. The principal factor responsible for the observed immune effect does not appear to be the availability or concentration of single essential amino acids but rather the composite effect of the specific amino acid distribution in the protein.
通过测量C3H/HeN小鼠对绵羊红细胞（SRBC）和马红细胞（HRBC）的特异性体液免疫反应，以及在用卡介苗（BCG）刺激后对植物血凝素（PHA）和刀豆蛋白A（Con A）的非特异性脾细胞反应性，研究了不同剂量的膳食乳白蛋白（L）、酪蛋白（C）、大豆蛋白（S）、小麦蛋白（W）和普瑞纳啮齿动物饲料（基础日粮）对其免疫反应性的影响。这些日粮的营养效率正常且相似。发现喂食L日粮的小鼠的免疫反应比喂食相应C日粮的小鼠高出近五倍。喂食C、S和W日粮的小鼠的体液免疫反应明显低于喂食基础日粮的小鼠，而喂食L日粮的小鼠的体液免疫反应则更高。所有测试蛋白质的上述免疫效果在浓度为20 g/100 g时即可获得，日粮中蛋白质含量为30 g/100 g和40 g/100 g时没有进一步增加。喂食L日粮的小鼠对PHA和Con A的丝裂原反应性仅略高于喂食C日粮的小鼠。喂食C、S或W蛋白日粮的小鼠之间的免疫反应差异不大。观察到的免疫效果的主要因素似乎不是单一必需氨基酸的可用性或浓度，而是蛋白质中特定氨基酸分布的综合作用。
