Bos H J, Schouten W J, Noordpool H, Makbin M, Oostburg B F
Am J Trop Med Hyg. 1980 May;29(3):358-63. doi: 10.4269/ajtmh.1980.29.358.
Sera from nine populations in Surinam, South America, were screened for antibodies against Entamoeba histolytica by the enzyme-linked immunosorbent assay (ELISA) technique and a precipitin test (gel diffusion or counterimmunoelectrophoresis). In two small surveys in 1974 and 1978, a high rate of clinically significant ELISA titers was found in a rather isolated group of Amerindians living near the Brazilian border, predominately in the youngest age groups. Amerindians living near the coast showed a low level of seropositivity. Low levels were also found in Bush Negro villages in the interior of Surinam and in Hindustani school children from the savannah area. A high frequency of ELISA titers greater than or equal to 320, indicating a high rate of invasive amebiasis, was detected in persons in a mental institution in Paramaribo. It is concluded that the ELISA, which is much more sensitive than the precipitin tests, is a useful screening test for assessing the prevalence of invasive amebiasis on an epidemiological scale.
采用酶联免疫吸附测定（ELISA）技术和沉淀试验（凝胶扩散或对流免疫电泳），对南美洲苏里南九个群体的血清进行了抗溶组织内阿米巴抗体筛查。在1974年和1978年的两项小型调查中，在居住在巴西边境附近的一个相对孤立的美洲印第安人群体中发现了较高比例具有临床意义的ELISA滴度，主要集中在最年轻的年龄组。居住在海岸附近的美洲印第安人血清阳性水平较低。在苏里南内陆的丛林黑人村庄以及来自大草原地区的印度斯坦学童中也发现了较低水平。在帕拉马里博的一家精神病院里，检测到ELISA滴度大于或等于320的频率很高，这表明侵袭性阿米巴病的发病率很高。得出的结论是，ELISA比沉淀试验灵敏得多，是在流行病学规模上评估侵袭性阿米巴病患病率的一种有用筛查试验。
