通过阻抗法对肉类中大肠菌群进行定量估计的快速方法的评估。
Evaluation of a rapid method for the quantitative estimation of coliforms in meat by impedimetric procedures.
作者信息
Martins S B, Selby M J
出版信息
Appl Environ Microbiol. 1980 Mar;39(3):518-24. doi: 10.1128/aem.39.3.518-524.1980.
A 24-h instrumental procedure is described for the quantitative estimation of coliforms in ground meat. The method is simple and rapid, and it requires but a single sample dilution and four replicates. The data are recorded automatically and can be used to estimate coliforms in the range of 100 to 10,000 organisms per g. The procedure is an impedance detection time (IDT) method using a new medium, tested against 131 stock cultures, that markedly enhances the impedance response of gram-negative organisms, and it is selective for coliforms. Seventy samples of ground beef were analyzed for coliforms by the IDT method and the conventional three-dilution, two-step most-probable-number test tube procedure. Seventy-nine percent of the impedimetric estimates fell within the 95% confidence limits of the most-probable-number values. This corresponds to the criteria used to evaluate other coliform tests, with the added advantage of a single dilution and more rapid results.
本文描述了一种用于定量测定碎肉中大肠菌群的24小时仪器检测方法。该方法简单快速，仅需一次样品稀释和四个重复样本。数据自动记录，可用于估计每克含100至10000个微生物范围内的大肠菌群。该方法是一种阻抗检测时间（IDT）方法，使用一种新培养基，对131种菌株进行了测试，该培养基显著增强了革兰氏阴性菌的阻抗响应，且对大肠菌群具有选择性。采用IDT方法和传统的三步稀释、两步最可能数试管法对70份碎牛肉样品进行大肠菌群分析。79%的阻抗法估计值落在最可能数法值的95%置信区间内。这符合用于评估其他大肠菌群检测方法的标准，且具有单次稀释和结果更快速的额外优势。
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