Gabrion J, Travers F, Benyamin Y, Sentein P, Van Thoai N
Cell Biol Int Rep. 1980 Jan;4(1):59-68. doi: 10.1016/0309-1651(80)90010-7.
In thyroid cells (rat or hog), actin has been detected by immunofluorescence with an antiactin antibody and, in electron microscopy by decoration "in situ" with heavy meromyosin. The antibody as the heavy meromyosin method have shown that actin microfilaments are especially localized at the apical pole of the cells, in a region where thin filaments are usually observed by conventional methods of electron microscopy. These microfilaments are attached to the apical membrane at the ends of the microvilli and form dense bundles at their cores. They are polarized towards the interior of the cell. Decorated filaments are also organized in a clear network, parallel to the apical membrane; they are associated with microvillar bundles, but also with small apical vesicles and lateral membranes, in tight or gap junctions.
在甲状腺细胞（大鼠或猪）中，通过用抗肌动蛋白抗体进行免疫荧光检测以及在电子显微镜下用重酶解肌球蛋白进行“原位”标记检测到了肌动蛋白。抗体和重酶解肌球蛋白方法均表明，肌动蛋白微丝特别定位于细胞的顶端极，在该区域通过传统电子显微镜方法通常可观察到细肌丝。这些微丝在微绒毛末端附着于顶端膜，并在其核心形成致密束。它们向细胞内部极化。标记的细丝也以与顶端膜平行的清晰网络形式排列；它们与微绒毛束相关，但也与紧密或间隙连接中的小顶端囊泡和侧膜相关。
