受精海胆卵微绒毛内的肌动蛋白丝极化束。
Polarized bundles of actin filaments within microvilli of fertilized sea urchin eggs.
作者信息
Burgess D R, Schroeder T E
出版信息
J Cell Biol. 1977 Sep;74(3):1032-7. doi: 10.1083/jcb.74.3.1032.
Abstract
We report on the internal ultrastructure of long, finger-like microvilli which cover the surface of the fertilized sea urchin egg. Eggs were attached to polylysine-coated surfaces; their upper portions were sheared away with a stream of buffer which left behind only their plasma membranes and adjacent cytoplasmic structures. Scanning electron microscopy (EM) of such fragments revealed intact thin protoplasmic projections radiating away from the body of the cortex. By transmission EM of cortices similarly prepared on grids, small bundles of microfilaments appear as cores within the thin cytoplasmic projections. These microfilaments are shown to be composed of actin by their ability to interact with muscle heavy meromyosin (HMM). HMM-decorated microfilaments possess repeating arrowheads which uniformly point toward the cell interior. Actin bundles in the microvilli of sea urchin eggs may mediate microvillus support and elongation.
摘要
我们报告了覆盖受精海胆卵表面的长指状微绒毛的内部超微结构。将卵附着在聚赖氨酸包被的表面；用缓冲液流将其上部剪掉，仅留下它们的质膜和相邻的细胞质结构。对这些片段进行扫描电子显微镜（EM）观察，发现完整的薄原生质突起从皮质主体向外辐射。通过在网格上类似制备的皮质进行透射电镜观察，小束微丝在薄细胞质突起内呈现为核心。这些微丝通过与肌肉重酶解肌球蛋白（HMM）相互作用的能力，被证明是由肌动蛋白组成的。用HMM标记的微丝具有重复的箭头，它们一致地指向细胞内部。海胆卵微绒毛中的肌动蛋白束可能介导微绒毛的支撑和伸长。
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