Sykes A, Chard T
Clin Chem. 1980 Jul;26(8):1224-6.
We describe an immunoassay for human pregnancy-specific beta 1-glycoprotein by use of fluorescein-labeled antibodies to this protein. The technique is generally similar to that of a "two-site" immunoradiometric assay but has the advantage of stability of tracer and avoidance of radiation hazard. However, the procedure requires relatively large quantities of antibody. Furthermore, the within-assay coefficient of variation (11.8%) is greater than that for radioimmunoassay (6.5%). This results from imprecision of the end-point detection, and some inherent difficulties in optimizing a "two-site" immunometric system. Concentrations of endogenous material as measured by the fluorometric assay are 1.9-fold higher than those obtained with radioimmunoassay, probably because the former detects multiple forms of this heterogeneous material.
我们描述了一种使用针对人妊娠特异性β1-糖蛋白的荧光素标记抗体进行的免疫测定法。该技术总体上类似于“双位点”免疫放射测定法，但具有示踪剂稳定性好且避免辐射危害的优点。然而，该方法需要相对大量的抗体。此外，测定内变异系数（11.8%）高于放射免疫测定法（6.5%）。这是由于终点检测不精确以及在优化“双位点”免疫测定系统时存在一些固有困难所致。荧光测定法测得的内源性物质浓度比放射免疫测定法测得的高1.9倍，可能是因为前者检测到这种异质性物质的多种形式。
