[Radioimmunoassay of SP1 in obstetrics and gynecological oncology].

The authors by means of radioimmunoassay analyze SP1 values in selected obstetric and oncological samples. Seven amniotic fluid samples showed various degrees of positivity, but not strictly correlated to the gestational age (previous analysis by immunodiffusion were always negative). SP1 concentrations in ectopic pregnancy confermed similar investigations by other authors. In the oncological field only one serum was highly positive (440 ng/ml); negativity of others could be due to the long time of storage. The SP1 was assayed using a sperimental kit supplied by Boehringwerke A.G. (Marburg, W.G..). Assay procedure: preparation of a standard curve (7 - 440 ng/ml) from which the unknown SP1 content was determinated. Reaction time: 16 - 24 h/20 degrees C (first incubation) and 0.5 - 4 h/20 degrees C (second incubation).