Björntorp P, Enzi G, Karlsson M, Kral J, Larsson B, Sjöström L, Smith U
Int J Obes. 1980;4(1):11-9.
Glucose metabolism in fat cells from fasted-refed rats and ad-libitum-fed controls were studied in the postabsorptive state. Two types of experiments were performed. First, each rat donated one epididymal fat pad and the metabolic results were expressed in relation to the number and size of fat cells of these tissues. Second, the other epididymal fat pad in the rats in each experimental group was pooled for experiments in duplicate where liberated fat cells were separated in fat-cell size classes, which enabled comparisons of metabolic activities at the same fat-cell size. Glucose incorporation into carbon dioxide and triglyceride glycerol and fatty acids were about equally elevated in absolute terms in the refed rats. In relative terms the increase was much more pronounced in carbon dioxide and fatty acids because these activities were very low in control rats. These results confirm previous results showing elevated metabolism after fasting-refeeding, and also demonstrate that this is an adaptation of fat-cell metabolism rather than a consequence of a higher cellular density of adipose tissue after fasting-refeeding with smaller fat cells. Adaptations of metabolism after fasting-refeeding might be of potential importance for elucidation of the cause of the rapid relapse after weight decrease of obese subjects with diminished fat-cell size.
在吸收后状态下，研究了禁食再喂养大鼠和自由进食对照大鼠脂肪细胞中的葡萄糖代谢。进行了两种类型的实验。首先，每只大鼠捐献一个附睾脂肪垫，代谢结果以这些组织中脂肪细胞的数量和大小来表示。其次，将每个实验组大鼠的另一个附睾脂肪垫合并进行重复实验，将分离出的脂肪细胞按脂肪细胞大小分类，从而能够比较相同脂肪细胞大小下的代谢活性。就绝对值而言，再喂养大鼠中葡萄糖掺入二氧化碳、甘油三酯甘油和脂肪酸的量均约有同等程度的升高。就相对值而言，二氧化碳和脂肪酸的增加更为显著，因为这些活性在对照大鼠中非常低。这些结果证实了先前的结果，即禁食再喂养后代谢升高，并且还表明这是脂肪细胞代谢的一种适应，而不是禁食再喂养后脂肪细胞变小导致脂肪组织细胞密度更高的结果。禁食再喂养后代谢的适应对于阐明脂肪细胞大小减小的肥胖受试者体重下降后迅速复发的原因可能具有潜在重要性。
