Gonda M A, Gregg M, Elser J E, Hsu K C
J Histochem Cytochem. 1980 Jul;28(7):710-3. doi: 10.1177/28.7.6993555.
The sensitivity and specificity of the unlabeled antibody-hemocyanin bridge method for immunoelectron microscopic localization of virion and cell surface antigens has been demonstrated using a hyperimmune serum to the Rauscher murine leukemia virus structural envelope glycoprotein (gp 70). The technique localized the gp70 on the viral envelope and on the infected cell surface at dilutions of greater than 10(-3) for the primary antiviral serum. Little or no nonspecific binding of hemocyanin was detected in control experiments using high concentrations of normal nonreacting or adequately absorbed antiviral primary sera and excess antibody bridge and marker; thus, the system is highly specific. Furthermore, sensitivity can be increased approximately fivefold by marked amplification steps whereby a specimen fixing only a slight amount of hemocyanin can be subsequently treated with antihemocyanin antibody, followed by hemocyanin.
使用针对劳氏鼠白血病病毒结构包膜糖蛋白（gp70）的超免疫血清，已证明了未标记抗体 - 血蓝蛋白桥接法在免疫电子显微镜下定位病毒体和细胞表面抗原的敏感性和特异性。该技术可在高于10（-3）稀释度的抗病毒原血清中，将gp70定位在病毒包膜和受感染细胞表面。在使用高浓度正常无反应或充分吸收的抗病毒原血清以及过量抗体桥和标记物的对照实验中，未检测到血蓝蛋白的非特异性结合；因此，该系统具有高度特异性。此外，通过显著的放大步骤可使敏感性提高约五倍，即对仅固定少量血蓝蛋白的标本，随后用抗血蓝蛋白抗体处理，再加入血蓝蛋白。
