Landini M P, Severi B, Badiali L, Gonczol E, Mirolo G
Institute of Microbiology, University of Bologna, Italy.
Intervirology. 1987;27(3):154-60. doi: 10.1159/000149734.
The localization of the major cytomegalovirus glycoprotein, both in the viral particle and in the cell membrane, was studied by means of indirect immunofluorescence and immunoelectron microscopy using a guinea pig anti-glycoprotein hyperimmune serum recently obtained by Gonczol et al. [1986]. By indirect immunofluorescence a slight and uneven positivity was observed on the plasma membrane of unfixed cells starting from 72 h postinfection (p.i.) until the end of our observation time (7 days p.i.). At immunoelectron microscopy the plasma membrane proved positive only where the virus and dense bodies budded through the membrane to form their own envelope. Extracellular viral particles (both viruses and dense bodies) appeared very strongly labeled on the external surface.
利用刚由贡佐尔等人[1986年]获得的豚鼠抗糖蛋白超免疫血清，通过间接免疫荧光法和免疫电子显微镜技术，研究了巨细胞病毒主要糖蛋白在病毒颗粒和细胞膜中的定位。通过间接免疫荧光法观察到，从感染后72小时（p.i.）直到我们观察期结束（感染后7天），未固定细胞的质膜上出现轻微且不均匀的阳性反应。在免疫电子显微镜下，仅在病毒和致密体通过膜出芽形成自身包膜的部位，质膜显示为阳性。细胞外病毒颗粒（病毒和致密体）在外表面呈现出非常强烈的标记。
