Molenaar J, Van de Grind W A
J Neurosci Methods. 1980 Apr;2(2):135-52. doi: 10.1016/0165-0270(80)90055-2.
A method is described for recording stereotaxically from single retinal neurons in the optically intact in vivo eye of the cat. The method is implemented with the help of a new type of stereotaxic instrument and a specially developed stereotaxic atlas of the cat's eye and retina. The instrument is extremely stable and facilitates intracellular recording from retinal neurons. The microelectrode can be rotated about two mutually perpendicular axes, which intersect in the freely positionable pivot point of the electrode manipulation system. When the pivot point is made to coincide with a small electrode-entrance hole in the sclera of the eye, a large retinal region can be reached through this fixed hole in the immobilized eye. The stereotaxic method makes it possible to choose a target point on the presented eye atlas and predict the settings of the instrument necessary to reach this target. This method also includes the prediction of the corresponding light stimulus position on a tangent screen and the calculation of the projection of the recording electrode on this screen. The sources of error in the method were studied experimentally and a numerical perturbation analysis was carried out to study the influence of each of the sources of error on the final result. The overall accuracy of the method is of the order of 5 degrees of visual angle, which will be sufficient for most purposes.
本文描述了一种在猫的光学完整的活体眼内对单个视网膜神经元进行立体定位记录的方法。该方法借助一种新型立体定位仪器以及专门开发的猫眼和视网膜立体定位图谱得以实现。该仪器极其稳定，便于对视网膜神经元进行细胞内记录。微电极可围绕两个相互垂直的轴旋转，这两个轴在电极操纵系统的可自由定位的枢轴点相交。当枢轴点与眼巩膜上的一个小电极入口孔重合时，通过固定在固定眼上的这个孔，可以到达较大的视网膜区域。立体定位方法使得能够在所呈现的眼图谱上选择一个目标点，并预测到达该目标所需的仪器设置。该方法还包括预测切线屏上相应光刺激的位置以及计算记录电极在该屏幕上的投影。通过实验研究了该方法中的误差来源，并进行了数值微扰分析，以研究每个误差来源对最终结果的影响。该方法的总体精度约为5度视角，这对于大多数目的来说已经足够。
