Baker R S, Bonin A M, Stupans I, Holder G M
Mutat Res. 1980 Jun;71(1):43-52. doi: 10.1016/0027-5107(80)90005-6.
A highly significant enhancement of mutagenicity occurs with 11 polycyclic aromatic hydrocarbons when 3-methylcholanthrene-induced guinea pig liver S9 is substituted for Aroclor-induced rat liver S9 in the Ames test. The use of MC-induced guinea pig liver S9 is particularly valuable for detecting the weak mutagenicity of benz[c]acridine, which is barely positive in a standard Ames assay. However, anthracene and phenanthrene, which are generally considered not to be carcinogens, remain non-mutagenic for strain TA100. This enhancement of mutagenicity does not correlate with arylhydrocarbon hydroxylase activities of the various liver preparations and does not apply to certain other non-PAH mutagens, including beta-naphthylamine, aflatoxin B1 and 4-dimethylaminoazobenzene.
在艾姆斯试验中，当用3-甲基胆蒽诱导的豚鼠肝脏S9替代多氯联苯诱导的大鼠肝脏S9时，11种多环芳烃的致突变性显著增强。使用3-甲基胆蒽诱导的豚鼠肝脏S9对于检测苯并[c]吖啶的弱致突变性特别有价值，苯并[c]吖啶在标准艾姆斯试验中几乎呈阴性。然而，通常认为不是致癌物的蒽和菲对TA100菌株仍然无致突变性。这种致突变性的增强与各种肝脏制剂的芳烃羟化酶活性无关，并且不适用于某些其他非多环芳烃诱变剂，包括β-萘胺、黄曲霉毒素B1和4-二甲基氨基偶氮苯。
