Tomsíková A, Sach J, Zavázal V, Masler L, Nováckova D
Mycopathologia. 1980 Jul 1;71(2):103-11. doi: 10.1007/BF00440616.
Attempts were made to prepare a sensitive antigen from C. albicans suitable for detecting humoral antibodies and hypersensitivity in deep-seated candidiasis, in patients at risk of invasive candidiasis and in allergic states caused by Candida. 5343 persons suffering from systemic, bronchial, vaginal candidiasis, bronchial asthma, chronic bronchitis, polyarthritis nodosa, ulcus cruris, malignancy, rhinitis pollinosa, vasomotorica, and non infected miners, farmers and blood donors were investigated on the presence of antibodies and hypersensitivity against 8 different antigen preparations. The extracellular protein and mannan- protein isolated from the cultivation medium of C. albicans proved the most sensitive for specific anticandida antibodies. The mannan, especially the mannan isolated from the cell surface of C. albicans determined best for the allergy. Comparison made of commerical Candidine showed similar activity. The whole cell C. albicans antigens as well as the mixed Candida antigen reacted much weakly. Comparison made of autoantigen, C. albicans and mixed C. albicans antigen proved the highest sensitivity of the autoantigen.
人们试图从白色念珠菌中制备一种敏感抗原，用于检测深部念珠菌病、侵袭性念珠菌病高危患者以及由念珠菌引起的过敏状态下的体液抗体和超敏反应。对5343名患有系统性、支气管、阴道念珠菌病、支气管哮喘、慢性支气管炎、结节性多关节炎、小腿溃疡、恶性肿瘤、花粉性鼻炎、血管舒缩性鼻炎的患者以及未受感染的矿工、农民和献血者，就其针对8种不同抗原制剂的抗体和超敏反应情况进行了调查。从白色念珠菌培养基中分离出的细胞外蛋白和甘露聚糖蛋白对特异性抗念珠菌抗体最为敏感。甘露聚糖，尤其是从白色念珠菌细胞表面分离出的甘露聚糖，对过敏反应的测定效果最佳。对市售念珠菌素的比较显示出类似的活性。白色念珠菌全细胞抗原以及混合念珠菌抗原的反应则弱得多。对自身抗原、白色念珠菌和混合白色念珠菌抗原的比较证明自身抗原具有最高的敏感性。
