基因重组中的同源配对：RecA蛋白与解螺旋蛋白共同作用形成D环。
Homologous pairing in genetic recombination: formation of D loops by combined action of recA protein and a helix-destabilizing protein.
作者信息
Shibata T, DasGupta C, Cunningham R P, Radding C M
出版信息
Proc Natl Acad Sci U S A. 1980 May;77(5):2606-10. doi: 10.1073/pnas.77.5.2606.
Abstract
Escherichia coli single-strand binding protein (SSB) or phage T4 gene 32 protein reduced the amount of recA protein required to catalyze the formation of D loops from double-stranded DNA and homologous single-stranded fragments. Neither SSB nor gene 32 protein alone catalyzed the formation of D loops, and excessive amounts of either protein, amounts that were sufficient to saturate the single strands, inhibited the formation of D loops completely. Both the stimulatory activity and the inhibitory activity of SSB resisted boiling, which is consistent with the known thermal stability of SSB, whereas the gene 32 protein was inactivated by heating. The formation of D loops in the presence of both recA protein and SSB required homologous DNA and ATP. Spermidine aided the combined action of SSB and recA protein in forming D loops, but Mg2+ alone was sufficient as a counterion.
摘要
大肠杆菌单链结合蛋白（SSB）或噬菌体T4基因32蛋白减少了催化双链DNA与同源单链片段形成D环所需的RecA蛋白量。单独的SSB或基因32蛋白均不能催化D环的形成，并且过量的这两种蛋白（足以使单链饱和的量）会完全抑制D环的形成。SSB的刺激活性和抑制活性均能抵抗煮沸，这与已知的SSB热稳定性一致，而基因32蛋白加热后会失活。在RecA蛋白和SSB同时存在的情况下，D环的形成需要同源DNA和ATP。亚精胺有助于SSB和RecA蛋白共同作用形成D环，但仅Mg2+作为抗衡离子就足够了。
相似文献
1
Homologous pairing in genetic recombination: formation of D loops by combined action of recA protein and a helix-destabilizing protein.基因重组中的同源配对：RecA蛋白与解螺旋蛋白共同作用形成D环。
Proc Natl Acad Sci U S A. 1980 May;77(5):2606-10. doi: 10.1073/pnas.77.5.2606.
2
Homologous pairing in genetic recombination. The pairing reaction catalyzed by Escherichia coli recA protein.遗传重组中的同源配对。由大肠杆菌recA蛋白催化的配对反应。
J Biol Chem. 1981 Jul 25;256(14):7565-72.
3
4
Effects of various single-stranded-DNA-binding proteins on reactions promoted by RecA protein.各种单链DNA结合蛋白对RecA蛋白所促进反应的影响。
J Bacteriol. 1987 Aug;169(8):3422-8. doi: 10.1128/jb.169.8.3422-3428.1987.
5
Hydrolysis of ATP dependent on homologous double-stranded DNA and single-stranded fragments promoted by RecA protein of Escherichia coli.大肠杆菌RecA蛋白促进的依赖同源双链DNA和单链片段的ATP水解。
J Biochem. 1982 May;91(5):1767-75. doi: 10.1093/oxfordjournals.jbchem.a133869.
6
RecBCD-dependent joint molecule formation promoted by the Escherichia coli RecA and SSB proteins.由大肠杆菌RecA和SSB蛋白促进的RecBCD依赖性联合分子形成。
Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3367-71. doi: 10.1073/pnas.88.8.3367.
7
Homologous pairing and topological linkage of DNA molecules by combined action of E. coli RecA protein and topoisomerase I.大肠杆菌RecA蛋白与拓扑异构酶I共同作用实现DNA分子的同源配对和拓扑连接
Cell. 1981 Apr;24(1):213-23. doi: 10.1016/0092-8674(81)90517-1.
8
Effects of the Escherichia coli SSB protein on the binding of Escherichia coli RecA protein to single-stranded DNA. Demonstration of competitive binding and the lack of a specific protein-protein interaction.大肠杆菌单链结合蛋白（SSB）对大肠杆菌重组蛋白A（RecA）与单链DNA结合的影响。竞争性结合的证明及特定蛋白质-蛋白质相互作用的缺失。
J Mol Biol. 1987 Jan 5;193(1):81-95. doi: 10.1016/0022-2836(87)90629-2.
9
Role of superhelicity in homologous pairing of DNA molecules promoted by Escherichia coli recA protein.超螺旋在大肠杆菌recA蛋白促进的DNA分子同源配对中的作用。
J Biol Chem. 1982 Jan 10;257(1):370-6.
10
Homologous pairing in genetic recombination: complexes of recA protein and DNA.基因重组中的同源配对：recA蛋白与DNA复合物
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5100-4. doi: 10.1073/pnas.76.10.5100.
引用本文的文献
1
Nonfilament-forming RecA dimer catalyzes homologous joint formation.非丝状体形成的 RecA 二聚体催化同源连接形成。
Nucleic Acids Res. 2018 Nov 16;46(20):10855-10869. doi: 10.1093/nar/gky877.
2
RecA-SSB Interaction Modulates RecA Nucleoprotein Filament Formation on SSB-Wrapped DNA.RecA-SSB 相互作用调节 RecA 核蛋白丝在 SSB 包裹 DNA 上的形成。
Sci Rep. 2017 Sep 19;7(1):11876. doi: 10.1038/s41598-017-12213-w.
3
A general solution for opening double-stranded DNA for isothermal amplification.用于等温扩增的双链DNA解链通用解决方案。
Sci Rep. 2016 Sep 30;6:34582. doi: 10.1038/srep34582.
4
Vital roles of the second DNA-binding site of Rad52 protein in yeast homologous recombination.Rad52 蛋白第二个 DNA 结合位点在酵母同源重组中的重要作用。
J Biol Chem. 2011 May 20;286(20):17607-17. doi: 10.1074/jbc.M110.216739. Epub 2011 Mar 28.
5
Analyses of six homologous proteins of Protochlamydia amoebophila UWE25 encoded by large GC-rich genes (lgr): a model of evolution and concatenation of leucine-rich repeats.对由富含GC的大基因（lgr）编码的嗜阿米巴原衣原体UWE25六种同源蛋白的分析：富含亮氨酸重复序列的进化与串联模型
BMC Evol Biol. 2007 Nov 16;7:231. doi: 10.1186/1471-2148-7-231.
6
The AAA+ protein ATAD3 has displacement loop binding properties and is involved in mitochondrial nucleoid organization.AAA+蛋白ATAD3具有置换环结合特性，并参与线粒体类核组织。
J Cell Biol. 2007 Jan 15;176(2):141-6. doi: 10.1083/jcb.200609158. Epub 2007 Jan 8.
7
Bacillus subtilis RecU Holliday-junction resolvase modulates RecA activities.枯草芽孢杆菌RecU霍利迪连接体解离酶调节RecA活性。
Nucleic Acids Res. 2005 Jul 15;33(12):3942-52. doi: 10.1093/nar/gki713. Print 2005.
8
Role of Saccharomyces single-stranded DNA-binding protein RPA in the strand invasion step of double-strand break repair.酿酒酵母单链DNA结合蛋白RPA在双链断裂修复的链侵入步骤中的作用。
PLoS Biol. 2004 Jan;2(1):E21. doi: 10.1371/journal.pbio.0020021. Epub 2004 Jan 20.
9
Homologous genetic recombination as an intrinsic dynamic property of a DNA structure induced by RecA/Rad51-family proteins: a possible advantage of DNA over RNA as genomic material.同源基因重组作为由RecA/Rad51家族蛋白诱导的DNA结构的一种内在动态特性：DNA作为基因组物质相对于RNA的一种可能优势。
Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8425-32. doi: 10.1073/pnas.111005198.
10
RecA protein promotes the regression of stalled replication forks in vitro.RecA蛋白在体外促进停滞复制叉的回归。
Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8211-8. doi: 10.1073/pnas.131022698.
本文引用的文献
1
Thermal renaturation of deoxyribonucleic acids.脱氧核糖核酸的热复性
J Mol Biol. 1961 Oct;3:585-94. doi: 10.1016/s0022-2836(61)80023-5.
2
Homologous pairing in genetic recombination: recA protein makes joint molecules of gapped circular DNA and closed circular DNA.遗传重组中的同源配对：RecA蛋白使缺口环状DNA和闭环状DNA形成联合分子。
Cell. 1980 May;20(1):223-35. doi: 10.1016/0092-8674(80)90250-0.
3
recA protein-catalyzed strand assimilation: stimulation by Escherichia coli single-stranded DNA-binding protein.RecA蛋白催化的链同化作用：受大肠杆菌单链DNA结合蛋白的刺激
Proc Natl Acad Sci U S A. 1980 Feb;77(2):857-61. doi: 10.1073/pnas.77.2.857.
4
Effects of the conformation of single-stranded DNA on renaturation and aggregation.单链DNA构象对复性和聚集的影响。
J Mol Biol. 1969 Apr;41(2):199-209. doi: 10.1016/0022-2836(69)90385-4.
5
Kinetics of renaturation of DNA.DNA复性动力学
J Mol Biol. 1968 Feb 14;31(3):349-70. doi: 10.1016/0022-2836(68)90414-2.
6
T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA.T4噬菌体基因32：DNA复制与重组中的一种结构蛋白。
Nature. 1970 Sep 26;227(5265):1313-8. doi: 10.1038/2271313a0.
7
Molecular recombination in T4 bacteriophage deoxyribonucleic acid. II. Single-strand breaks and exposure of uncomplemented areas as a prerequisite for recombination.T4噬菌体脱氧核糖核酸中的分子重组。II. 单链断裂和未互补区域的暴露作为重组的前提条件。
J Virol. 1967 Dec;1(6):1193-202. doi: 10.1128/JVI.1.6.1193-1202.1967.
8
Models of genetic recombination.基因重组模型。
Annu Rev Microbiol. 1974;28(0):445-68. doi: 10.1146/annurev.mi.28.100174.002305.
9
10
DNA synthesis on a double-stranded DNA template by the T4 bacteriophage DNA polymerase and the T4 gene 32 DNA unwinding protein.T4噬菌体DNA聚合酶和T4基因32解旋蛋白在双链DNA模板上进行DNA合成。
J Biol Chem. 1974 Sep 10;249(17):5668-76.
Zotero 插件