Cohen E
Ann Clin Lab Sci. 1980 May-Jun;10(3):258-62.
A review of recently published work is presented on the nature of the agglutination of human peripheral lymphocytes and cultured lymphoblastoid cells by the hemolymph agglutinins of two arachnoids, the Horsefoot (Limulus polyphemus), alias Horseshoe Crab, and the Saharan Scorpion (Androctonus australis). Microagglutination techniques were utilized to study the specificity of the whole serum and purified agglutinin(s) of Limulus and the whole serum agglutinin(s) of Androctonus. Peripheral human lymphocytes of patients with chronic lymphocytic leukemia (CLL) gave higher agglutination titers and scores than did normal cells, with both species of invertebrate agglutinins. However, human B cells gave higher, although overlapping degrees of agglutination, than T cells. The major cell membrane receptor site for agglutinins of both species was the sialic acid moiety, N-acetyl neuraminic acid. Selected literature on plant and invertebrate agglutinins (lectins) was reviewed. These lectins are underscored as valuable cell membrane probes of surface receptors of human lymphocyte subpopulations.
本文综述了近期发表的有关两种蛛形纲动物——鲎（美洲鲎）和撒哈拉蝎子（澳氏毒蝎）的血淋巴凝集素对人外周淋巴细胞和培养的淋巴母细胞样细胞凝集作用性质的研究工作。采用微量凝集技术研究了鲎全血清及纯化凝集素的特异性以及澳氏毒蝎全血清凝集素的特异性。慢性淋巴细胞白血病（CLL）患者的外周血淋巴细胞与正常细胞相比，在两种无脊椎动物凝集素作用下均呈现出更高的凝集滴度和凝集分数。然而，人B细胞的凝集程度高于T细胞，尽管两者有重叠。两种凝集素的主要细胞膜受体位点均为唾液酸部分，即N - 乙酰神经氨酸。本文还对植物和无脊椎动物凝集素（凝集素）的相关文献进行了综述。这些凝集素被强调为研究人淋巴细胞亚群表面受体的有价值的细胞膜探针。
