Eckfeldt J H, Light R T
Clin Chem. 1980 Aug;26(9):1278-80.
We describe a rapid, accurate, and precise two-point kinetic assay for ethylene glycol. The method involves use of a standard kit for ethanol determination with yeast alcohol dehydrogenase, and of a centrifugal analyzer. Alcohol dehydrogenase catalyzes the oxidation of ethylene glycol in a trichloroacetic acid-precipitated specimen; the resulting NADH production is monitored at 340 nm. The reaction rate is linear with concentration to 1.5 g of ethylene glycol per liter. Interference from methanol, ethanol, and isopropanol was easily recognized after a 30-min incubation at 100 degrees C. We believe that the method can be readily adaptable to any narrow-bandwidth, stable, temperature-controlled spectrophotometer and so should provide more widely for the prompt assessment of patients in whom ethylene glycol poisoning is suspected.
我们描述了一种用于乙二醇的快速、准确且精确的两点动力学分析方法。该方法使用用于乙醇测定的标准试剂盒和酵母乙醇脱氢酶，以及一台离心分析仪。乙醇脱氢酶催化在三氯乙酸沉淀的标本中乙二醇的氧化；在340nm处监测生成的NADH。反应速率与浓度呈线性关系，直至每升1.5克乙二醇。在100℃孵育30分钟后，很容易识别出甲醇、乙醇和异丙醇的干扰。我们认为该方法可轻松适用于任何窄带宽、稳定、温度可控的分光光度计，因此应为疑似乙二醇中毒患者的快速评估提供更广泛的应用。
