Gupta R N, Eng F, Gupta M L
Clin Chem. 1982 Jan;28(1):32-3.
In this novel procedure for determining ethylene glycol in plasma by liquid chromatography, benzoyl esters of ethylene glycol and of benzyl alcohol (used as the internal standard) are prepared directly in plasma. The benzoyl esters, highly ultraviolet-absorbing chromogens, are ideal compounds for analysis by reversed-phase liquid chromatography with methanol/water as the mobile phase. The benzoyl derivative of ethylene glycol is well separated from the derivative of the internal standard and from plasma constituents. The standard curve is linear to 400 mg of ethylene glycol per liter. As little as 10 mg of ethylene glycol per liter of plasma can be measured. Other commonly ingested alcohols do not interfere.
在这种通过液相色谱法测定血浆中乙二醇的新方法中，乙二醇和苯甲醇（用作内标）的苯甲酰酯直接在血浆中制备。苯甲酰酯是具有高紫外吸收的发色团，是用甲醇/水作为流动相进行反相液相色谱分析的理想化合物。乙二醇的苯甲酰衍生物与内标衍生物及血浆成分能很好地分离。标准曲线在每升含400毫克乙二醇时呈线性。每升血浆中低至10毫克的乙二醇都能被检测出来。其他常见摄入的醇类不产生干扰。
