Anderson D P, Dixon O W, Simon R C
Dev Biol Stand. 1980;45:157-62.
Biologics for serodiagnostic identification of fish diesease agents and for determination of efficacy and potency of fish bacterins are now being offered on a limited basis to governmental hatchery biologists and commercial aquaculture farms by the National Fish Health Research Laboratory - Leetown. Difficulties arise when biologics are used for determining standard parameters for fish bacterins because there may be variations in the fishes' immune response due to population differences or effects of temperature and pollutants in the aquatic environment. The passive hemolytic plaque assay and passive hemagglutination tests are used to determine efficacy and potency of bacterins delivered by different routes and among batches of bacterins against fish disease agents of Aeromonas salmonicida (furunculosis) and Yersinia ruckeri (redmouth). The kinetics of the immune response with a defined population held in a standardized environment is established. We are presently recommending the development of a rainbow trout strain registry so consistency can be maintained for the standardization of biologics for fishes.
国家鱼类健康研究实验室——利敦镇目前正在有限范围内，向政府孵化场生物学家和商业水产养殖场提供用于鱼类疾病病原体血清学诊断鉴定以及鱼类疫苗效力和效能测定的生物制品。当使用生物制品来确定鱼类疫苗的标准参数时会出现困难，因为由于种群差异或水生环境中温度和污染物的影响，鱼类的免疫反应可能会有所不同。被动溶血空斑试验和被动血凝试验用于确定不同给药途径以及不同批次疫苗针对杀鲑气单胞菌（疖疮病）和鲁氏耶尔森菌（红嘴病）等鱼类病原体的效力和效能。在标准化环境中饲养特定种群，建立免疫反应动力学。我们目前建议建立一个虹鳟鱼品系登记册，以便在鱼类生物制品标准化方面保持一致性。
