Cheng T P, Byrd F I, Whitaker J N, Wood J G
J Cell Biol. 1980 Aug;86(2):624-33. doi: 10.1083/jcb.86.2.624.
Immunocytochemistry has been used to study the distribution of the major 180,000-mol wt protein of coated vesicles in rodent cerebellum. An antibody to the coat protein was prepared in rabbits and characterized by immunodiffusion and immunofixation of polyacrylamide gels. At the light microscope level the protein was primarily localized in punctate profiles surrounding Purkinje cells and within the cerebellar glomeruli. At the electron microscope level the punctate distribution was confined to presynaptic terminals of basket and Golgi II neurons as well as mossy fiber terminals of the glomeruli. This label was heaviest on the lattice coat of coated vesicles but, in addition, label was found within the presynaptic axoplasm and along the cytoplasmic surface of the plasmalemma. Coated vesicles in cell somata were labeled as well as the cytosol around groupings of these vesicles. These data suggest that there may be two forms (or more) of coated vesicle protein in neurons, a lattice form associated with coated vesicles and a soluble form associated with the cytoplasmic matrix.
免疫细胞化学已被用于研究有被小泡中主要的180,000道尔顿蛋白质在啮齿动物小脑内的分布。用兔制备了针对包被蛋白的抗体，并通过聚丙烯酰胺凝胶免疫扩散和免疫固定法对其进行了鉴定。在光学显微镜水平，该蛋白质主要定位于围绕浦肯野细胞的点状结构以及小脑小球内。在电子显微镜水平，点状分布局限于篮状细胞和高尔基II型神经元的突触前终末以及小球的苔藓纤维终末。这种标记在有被小泡的晶格包被上最为明显，但此外，在突触前轴质内以及沿着质膜的胞质表面也发现了标记。细胞体中的有被小泡以及这些小泡聚集周围的胞质溶胶都有标记。这些数据表明，神经元中可能存在两种（或更多）形式的有被小泡蛋白，一种是与有被小泡相关的晶格形式，另一种是与细胞质基质相关的可溶性形式。
