Wood J G, Wallace R W, Whitaker J N, Cheung W Y
J Cell Biol. 1980 Jan;84(1):66-76. doi: 10.1083/jcb.84.1.66.
Antisera to calmodulin, a Ca2%-dependent modulator protein, and a heat-labile calmodulin-binding protein have been used to localize these proteins in mouse caudate-putamen. The two proteins appear to be located at identical sites in this brain area. At the light microscopic level, calmodulin and calmodulin-binding protein are found within the cytoplasm and processes of large cells. At the electron microscopic level the proteins are associated with neuronal elements only, primarily at postsynaptic sites within neuronal somata and dendrites. Within the dendrites the immunocytochemical label is associated predominantly with the postsynaptic density and dendritic microtubules. These results are in accord with recent biochemical and immunihistochemical studies of calmodulin in brain and in dividing cells. Thus, calmodulin and the heat-labile calmodulin-binding protein may play a role in the nervous system at the site of neurotransmitter action and at the level of microtubular function.
已使用针对钙调蛋白（一种依赖钙离子的调节蛋白）和一种热不稳定钙调蛋白结合蛋白的抗血清，在小鼠尾状核 - 壳核中定位这些蛋白。这两种蛋白在该脑区似乎位于相同位点。在光学显微镜水平，钙调蛋白和钙调蛋白结合蛋白存在于大细胞的细胞质和突起内。在电子显微镜水平，这些蛋白仅与神经元成分相关，主要位于神经元胞体和树突内的突触后位点。在树突内，免疫细胞化学标记主要与突触后致密物和树突微管相关。这些结果与最近关于脑和分裂细胞中钙调蛋白的生化及免疫组织化学研究一致。因此，钙调蛋白和热不稳定钙调蛋白结合蛋白可能在神经系统中神经递质作用位点及微管功能水平发挥作用。
