Nakane H, Nakane Y, Corvol P, Menard J
Kidney Int. 1980 May;17(5):607-14. doi: 10.1038/ki.1980.71.
Renin secretion was compared in vivo and in vitro among five groups of rats, each group subjected to a different sodium balance for 10 to 14 days. The state of the renin-angiotensin system in vivo was evaluated by measuring the renal renin (RR) and the plasma renin concentration (PRC) in both anesthetized and nonanesthetized animals. The in vitro renin secretion rate (RSR) was determined in isolated perfused kidneys. RR was reduced (-48%) by sodium loading and deoxycorticosterone (DOCA) and increased (+27%) by sodium deprivation and furosemide. Sodium loading and DOCA reduced both the PRC and the RSR to less than 20% of control values. By contrast, sodium deprivation and furosemide induced a more than fourfold rise in the PRC but only a small increase in the RSR (+37%). These results indicate that changes in fractional renin release are induced by sodium balance variation, and these changes are preserved in vitro only in sodium-loaded states. The inability of sodium-deprived kidneys to maintain high renin release in vitro suggests that high plasma renin levels in these rats depend on mechanisms that are not preserved in vitro. There was no evidence supporting the participation of inactive renin secretion in the regulation of fractional renin release under varying sodium balance.
在五组大鼠中对体内和体外的肾素分泌进行了比较，每组大鼠在不同的钠平衡状态下维持10至14天。通过测量麻醉和未麻醉动物的肾肾素（RR）和血浆肾素浓度（PRC）来评估体内肾素-血管紧张素系统的状态。在离体灌注肾中测定体外肾素分泌率（RSR）。钠负荷和脱氧皮质酮（DOCA）使RR降低（-48%），而钠缺失和速尿使RR升高（+27%）。钠负荷和DOCA使PRC和RSR均降至对照值的20%以下。相比之下，钠缺失和速尿使PRC升高超过四倍，但RSR仅小幅增加（+37%）。这些结果表明，肾素释放分数的变化是由钠平衡变化引起的，且这些变化仅在钠负荷状态下在体外得以保留。钠缺失的肾脏在体外无法维持高肾素释放，这表明这些大鼠的高血浆肾素水平依赖于体外无法保留的机制。没有证据支持在不同钠平衡状态下，无活性肾素分泌参与肾素释放分数的调节。
