Noon K F, Rogul M, Binn L N, Keefe T J, Marchwicki R H, Appel M J
Am J Vet Res. 1980 Apr;41(4):605-9.
An enzyme-linked immunosorbent assay (ELISA) for canine immunoglobulin (Ig) G antibodies to canine distemper virus (CDV) was developed and its test results were compared with those of the serum-neutralization (SN) test. The sera of 273 random-source adult dogs were examined. The two tests had a high degree of correlation. Very few discrepancies occurred and most of these were at the lower limits of each test. When the sera were tested at 1:100 dilution, there was a 98% agreement between the ELISA and SN test. Titrated SN and IgG ELISA tests also were performed on sera from 77 dogs whose lifetime medical histories were known. The results showed excellent agreement between the tests. Only five of the 77 sera showed any discrepancies and these were at detection-threshold levels. To follow antibody development in two dogs experimentally infected with CDV, it was necessary to use an ELISA which detected both canine IgM and IgG. The ELISA detected CDV-specific IgM a week before the SN test results became positive. The IgM titers rose for 3 weeks and descended to lower levels about the 4th and the 5th weeks. The SN titers closely followed the IgM titers. The ELISA detected IgG antibody about the 5th and the 6th weeks of infection. Results of both the SN test and the IgG ELISA remained elevated through the 70th day of testing.
开发了一种用于检测犬瘟热病毒（CDV）的犬免疫球蛋白（Ig）G抗体的酶联免疫吸附测定（ELISA），并将其测试结果与血清中和（SN）试验的结果进行了比较。对273只随机来源的成年犬的血清进行了检测。这两种测试具有高度相关性。出现的差异很少，而且大多数差异出现在每种测试的下限。当血清以1:100稀释度进行检测时，ELISA和SN试验之间的一致性为98%。还对77只已知终生病史的犬的血清进行了滴定SN和IgG ELISA测试。结果表明这两种测试之间具有极好的一致性。77份血清中只有5份显示出任何差异，且这些差异处于检测阈值水平。为了跟踪两只实验感染CDV的犬的抗体产生情况，有必要使用一种能同时检测犬IgM和IgG的ELISA。ELISA在SN试验结果呈阳性前一周检测到CDV特异性IgM。IgM滴度上升3周，在第4周和第5周左右降至较低水平。SN滴度紧密跟随IgM滴度。ELISA在感染后第5周和第6周左右检测到IgG抗体。SN试验和IgG ELISA的结果在检测的第70天一直保持升高。
