使用酶联免疫吸附测定法对犬瘟热病毒感染进行血清学调查。

Serological survey of canine distemper virus infection using enzyme-linked immunosorbent assay.

作者信息

Gemma T, Miyashita N, Shin Y S, Okita M, Mori T, Iwatsuki K, Mikami T, Kai C

机构信息

Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.

出版信息

J Vet Med Sci. 1995 Aug;57(4):761-3. doi: 10.1292/jvms.57.761.

DOI:10.1292/jvms.57.761

PMID:8519915

Abstract

For detection of antibodies against canine distemper virus (CDV), an enzyme-linked immunosorbent assay (ELISA) was developed using a crude extract from cells infected with the Onderstepoort strain of CDV as antigen. Twenty-six sera from dogs experimentally vaccinated with the Snyder-Hill strain of CDV were compared by ELISA and a standard virus neutralization (NV) test. Since a good correlation between the titers obtained by both tests was observed, ELISA was considered to be a rapid and reliable method for a serological survey of CDV infection. When a total of 167 sera from dogs suspected of canine distemper under natural conditions were examined by the ELISA, 29 of the sera (17%) were found to have low VN antibody titers and high ELISA titers. The reason for the discrepancy in the titers was discussed.

摘要

为检测抗犬瘟热病毒（CDV）抗体，研发了一种酶联免疫吸附测定（ELISA）方法，该方法使用感染CDV Onderstepoort株的细胞粗提物作为抗原。通过ELISA和标准病毒中和（NV）试验对26份经实验接种CDV Snyder-Hill株的犬血清进行了比较。由于观察到两种试验获得的效价之间具有良好的相关性，ELISA被认为是一种用于CDV感染血清学调查的快速且可靠的方法。当用ELISA检测总共167份自然条件下疑似犬瘟热的犬血清时，发现其中29份血清（17%）的VN抗体效价低而ELISA效价高。对效价差异的原因进行了讨论。