Macknight A D
Fed Proc. 1980 Sep;39(11):2881-7.
The development of new techniques and modifications to existing methods are providing important information about cellular water and electrolytes in relation to transepithelial transport. Information obtained for one epithelium, the toad urinary bladder, from chemical analyses, isotopic studies, and electron microprobe analysis, is reviewed. Chemical and isotopic analyses allow estimation of average cellular water and ion contents and determination of the origin of ions from the media bathing the mucosal and basolateral cellular membranes. The electron microprobe provides additional information about the distributions of ions between the cell types that constitute the epithelial layer and also allows some measurement of ion distributions within individual cells. In toad urinary bladder epithelial cells there is broad agreement between the results obtained with these different techniques and a summary is provided of possible relationships between transepithelial sodium transport and cellular composition.
新技术的发展以及对现有方法的改进，正在提供有关细胞水和电解质与跨上皮运输关系的重要信息。本文综述了通过化学分析、同位素研究和电子微探针分析，从一种上皮组织——蟾蜍膀胱所获得的信息。化学和同位素分析能够估算细胞内水和离子的平均含量，并确定来自黏膜和基底外侧细胞膜周围介质中离子的来源。电子微探针提供了有关构成上皮层的不同细胞类型之间离子分布的额外信息，还能对单个细胞内的离子分布进行一些测量。在蟾蜍膀胱上皮细胞中，这些不同技术所获得的结果之间存在广泛共识，并总结了跨上皮钠运输与细胞组成之间可能的关系。
