Madri J A, Furthmayr H
Hum Pathol. 1980 Jul;11(4):353-66. doi: 10.1016/s0046-8177(80)80031-1.
The localization of type I, III, IV, and AB2 (V) collagens in normal and fibrotic human lungs was studied by indirect immunofluorescence techniques with affinity purified antibodies specific for these types of collagen. In the normal lung type I collagen appears to be localized to the interstitium of alveolar septa in irregular patterns. Type III appears to have a more prominent, irregular localization in septa and a perivascular localization. Types IV and V codistribute in linear patterns on alveolar and capillary basement membranes. Type V is also present in the interstitium. Dramatic changes in relative amounts and localization were noted in fibrotic lung samples by fluorescence microscopy. In fibrotic lung samples there is a marked increase in type I in thickened septae. Type III is markedly reduced and seen only in perivascular localization. Type V is markedly increased in the interstitium and located in areas of smooth muscle cell proliferation. No apparent change is noted in amount or localization of type IV collagen. These findings correlate well with biochemical data relating to the amounts of various collagens isolated by enzymatic extraction techniques. These results are discussed with respect to staging and evolution of the fibrotic process.
采用针对这些类型胶原蛋白的亲和纯化抗体，通过间接免疫荧光技术研究了I型、III型、IV型和AB2（V）型胶原蛋白在正常和纤维化人肺中的定位。在正常肺中，I型胶原蛋白似乎以不规则模式定位于肺泡间隔的间质。III型在间隔中似乎有更突出的、不规则的定位以及血管周围定位。IV型和V型在肺泡和毛细血管基底膜上呈线性共分布。V型也存在于间质中。通过荧光显微镜观察发现，纤维化肺样本中相对含量和定位有显著变化。在纤维化肺样本中，增厚间隔中的I型胶原蛋白显著增加。III型显著减少，仅见于血管周围定位。V型在间质中显著增加，并位于平滑肌细胞增殖区域。IV型胶原蛋白的数量或定位未观察到明显变化。这些发现与通过酶提取技术分离的各种胶原蛋白数量的生化数据密切相关。结合纤维化过程的分期和演变对这些结果进行了讨论。
