Suzuki S, Mitsunaga M, Miyoshi M, Hirakawa S, Nakagawa O, Miura H, Ofuji T
J Clin Endocrinol Metab. 1980 Sep;51(3):446-53. doi: 10.1210/jcem-51-3-446.
Antithyroglobulin (anti-Tg) antibodies cytophilic for human monocytes were detected in the serum of 30 of 45 patients with Hashimoto's thyroiditis using the passive rosette technique. These antibodies conferred on normal monocytes the ability to form rosettes with Tg-coated erythrocyres (E-Tg) in vitro. The percentage of E-Tg rosette-forming monocytes was correlated with serum anti-Tg antibody titers measured by tanned sheep red cell hemagglutination. Most serum cytophilic activities were recovered in the immunoglobulin G fraction and were not affected by heating to 56 C for 30 min or ultracentrifugation at 105,000 X g for 60 min. Passive E-Tg rosette formation by monocytes was immunologically specific and was inhibited by the addition of small amounts of free Tg into the medium but was not inhibited by the addition of normal human serum. The anti-Tg antibody-armed monocytes became cytotoxic against Tg-coated chicken erythrocytes and lysed target erythrocytes by an extracellular mechanism. It was suggested that monocytes might be armed by cytophilic antibodies in vivo, since monocytes of patients with Hashimoto's thyroiditis showed increased E-Tg binding (rosette formation) relative to monocytes from control subjects. These findings support the possible pathogenetic involvement of monocytes in human autoimmune thyroiditis.
采用被动玫瑰花结技术，在45例桥本甲状腺炎患者中的30例血清中检测到了对人单核细胞具有亲嗜性的抗甲状腺球蛋白（抗Tg）抗体。这些抗体使正常单核细胞在体外具有与包被甲状腺球蛋白的红细胞（E-Tg）形成玫瑰花结的能力。E-Tg玫瑰花结形成单核细胞的百分比与用鞣酸处理的绵羊红细胞血凝法测得的血清抗Tg抗体滴度相关。大多数血清亲嗜性活性存在于免疫球蛋白G组分中，且不受56℃加热30分钟或105,000×g超速离心60分钟的影响。单核细胞被动形成E-Tg玫瑰花结具有免疫特异性，向培养基中加入少量游离Tg可抑制其形成，但加入正常人血清则无抑制作用。抗Tg抗体武装的单核细胞对包被甲状腺球蛋白的鸡红细胞具有细胞毒性，并通过细胞外机制裂解靶红细胞。由于桥本甲状腺炎患者的单核细胞相对于对照受试者的单核细胞显示出E-Tg结合（玫瑰花结形成）增加，提示单核细胞可能在体内被亲嗜性抗体武装。这些发现支持了单核细胞可能参与人类自身免疫性甲状腺炎发病机制的观点。
