Miloserdova V D, Sukhorada E M
Tsitol Genet. 1980 May-Jun;14(3):19-22.
A continuous cell line (M. S. A. p.-1) was obtained from the ovaries of diapausing pupae of the oak silkmoth Antheraea pernyi. Active multiplication of cells was observed two months after preparing a primary culture from partly trypsinized tissues. Subcultures derived at this time were transmitted with 10 day intervals and endure over 30 passages. The cells are susceptible to the nuclear polyhedrosis viruses of Mamestra brassicae, Galleria mellonella, Malacosoma neustria and Bombyx mori.
从柞蚕滞育蛹的卵巢中获得了一个连续细胞系(M.S.A.p.-1)。用胰蛋白酶部分消化组织制备原代培养物两个月后,观察到细胞活跃增殖。此时获得的传代培养物每隔10天传代一次,已传代30多次。这些细胞对甘蓝夜蛾、大蜡螟、天幕毛虫和家蚕的核型多角体病毒敏感。