Wonitzki C
Arch Exp Veterinarmed. 1980;34(1):33-42.
An effort was made to characterise the O-antigen structure of three amino acid auxotrophic, attenuated, immunogenic smooth-shape mutants and of one wild strain of Salmonella typhimurium. Preliminary results are reported in this paper. Experiments were first undertaken with the view to determining strain-specific variation of germ yield following batch culturing as well as the movement of lipopolysaccharide levels along with the growth curve. Then culturing rules were prepared, with one and the same harvesting limit being specified (90 per cent of the strain-specific germ count) for widely comparable bacterial batches. The lipopolysaccharides obtained by phenolwater extraction from the above bacteria were characterised by gel chromatography, following their Freeman hydrolysis to degraded polysaccharide. Almost congruent polysaccharide chain peak patterns were obtained for all four strains, but despite their smooth nature all strains also contained rough polysaccharide portions without O-specific side chains. The findings so far obtained have not yet provided sufficient knowledge to discuss a possible correlation between reduced mutant virulence and qualitatively changed O-antigen structure in comparison to wild strains.
对鼠伤寒沙门氏菌的三个氨基酸营养缺陷型、减毒、免疫原性光滑型突变体以及一个野生菌株的O抗原结构进行了表征。本文报告了初步结果。首先进行实验，目的是确定分批培养后细菌产量的菌株特异性变化以及脂多糖水平随生长曲线的变化。然后制定培养规则，为广泛可比的细菌批次指定相同的收获极限（菌株特异性细菌计数的90%）。从上述细菌中通过酚水提取获得的脂多糖，在经弗里曼水解为降解多糖后，通过凝胶色谱进行表征。所有四个菌株都获得了几乎一致的多糖链峰型，但尽管它们具有光滑的性质，所有菌株也都含有没有O特异性侧链的粗糙多糖部分。到目前为止所获得的研究结果还没有提供足够的知识来讨论与野生菌株相比，突变体毒力降低与O抗原结构定性变化之间可能存在的相关性。
