蛋白质与来自大肠杆菌MRE 600核糖体亚基内RNA的放射化学交联。

Radiochemical cross-linking of proteins to RNA within ribosomal subunits from E. colil MRE 600.

作者信息

Giocanti N, Ekert B

出版信息

Int J Radiat Biol Relat Stud Phys Chem Med. 1980 Jul;38(1):63-82. doi: 10.1080/09553008014550951.

Abstract

Irradiation in vitro of 30S and 50S ribosomal subunits from E. coli MRE 600 by gamma rays from cobalt 60, in the absence of oxygen, results in the formation of covalent links between the RNAs and some ribosomal proteins. At low radiation doses, just sufficient to keep the integrity of the ribosome structure, the phenomenon appears highly specific. In the 30S irradiated particle, protein S1 attaches to 16S RNA; in the 50S irradiated particle, the proteins L3, L13, L19, L21, L22 and L24 are linked to 23S RNA. The mechanism of formation of these cross-links and their contribution to the study of the tridimensional ribosome structure are also discussed.

摘要

在无氧条件下，用钴60产生的伽马射线对来自大肠杆菌MRE 600的30S和50S核糖体亚基进行体外辐照，会导致RNA与一些核糖体蛋白之间形成共价连接。在低辐射剂量下，刚好足以保持核糖体结构的完整性，这种现象显得具有高度特异性。在受辐照的30S颗粒中，蛋白S1附着于16S RNA；在受辐照的50S颗粒中，蛋白L3、L13、L19、L21、L22和L24与23S RNA相连。还讨论了这些交联的形成机制及其对核糖体三维结构研究的贡献。