[通过γ射线辐照在大肠杆菌核糖体中形成RNA-蛋白质交联]
[Formation, by gamma irradiation, of RNA-protein cross-links in E. coli ribosomes].
作者信息
Ekert B, Giocanti N
出版信息
C R Acad Hebd Seances Acad Sci D. 1976 Mar 8;282(10):1053-6.
PMID:821634
Abstract
Gamma irradiation in desaerated conditions of E. coli MRE 600 ribosomes, labelled with C14 uracil, leads to a decrease of extractibility of C14 RNA by lithium chloride 4 M-urea 8 M. On the other hand, the radioactivity of the protein fraction increases with irradiation. These results strongly suggest that RNA-protein cross links are formed in irradiated ribosomes.
摘要
在无氧条件下，用C14尿嘧啶标记的大肠杆菌MRE 600核糖体进行伽马射线照射，会导致4M氯化锂-8M尿素对C14 RNA的提取率降低。另一方面，蛋白质组分的放射性随照射而增加。这些结果有力地表明，在受照射的核糖体中形成了RNA-蛋白质交联。
Zotero 插件