大肠杆菌的乙醛辅酶A脱氢酶
Acetaldehyde coenzyme A dehydrogenase of Escherichia coli.
作者信息
Clark D P, Cronan J E
出版信息
J Bacteriol. 1980 Oct;144(1):179-84. doi: 10.1128/jb.144.1.179-184.1980.
Abstract
Mutants of Escherichia coli (adh) in which alcohol dehydrogenase is derepressed under aerobic conditions were also found to overproduce acetaldehyde coenzyme a dehydrogenase. However, acetaldehyde coenzyme A dehydrogenase was induced by ethanol or acetaldehyde and subject to strong catabolite repression, whereas alcohol dehydrogenase was little affected by these conditions. Mutants no longer able to use ethanol as carbon source were isolated from an adh strain. Some of these mutants were revertants at the adh locus and no longer produced either alcohol dehydrogenase or acetaldehyde coenzyme A dehydrogenase. Others, designated acd, were found to lack only acetaldehyde coenzyme A dehydrogenase. The acd mutation was located at min 62 of the E. coli genetic map, the gene order being thyA-lysA-acd-serA-fda. Isolation of Tn10 insertions cotransducible with acd greatly simplified the mapping procedure.
摘要
在有氧条件下酒精脱氢酶被去阻遏的大肠杆菌（adh）突变体，也被发现过量产生乙醛辅酶A脱氢酶。然而，乙醛辅酶A脱氢酶由乙醇或乙醛诱导产生，并受到强烈的分解代谢物阻遏，而酒精脱氢酶受这些条件的影响很小。从adh菌株中分离出不再能够利用乙醇作为碳源的突变体。其中一些突变体是adh位点的回复突变体，不再产生酒精脱氢酶或乙醛辅酶A脱氢酶。其他的，被命名为acd，被发现仅缺乏乙醛辅酶A脱氢酶。acd突变位于大肠杆菌遗传图谱的62分钟处，基因顺序为thyA-lysA-acd-serA-fda。与acd共转导的Tn10插入片段的分离极大地简化了定位过程。
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