McGee J, Lanier J D
Ophthalmic Surg. 1980 Sep;11(9):609-10.
Donor corneal material stored or transported in M-K medium can freeze easily if placed in an insulated environment, such as a styrofoam container, with ice that is colder than 0 degrees C. To assure proper maintenance of the recommended temperature of M-K medium of + 4 degrees C, the ice used should be allowed to stand at room temperature until it has a glistening, wet look to its surfaces. M-K medium already cooled to + 4 degrees C can be safely stored and maintained at + 4 degrees C in a well-insulated styrofoam container for approximately 24 hours if this type of glistening-wet, melting ice is used.
如果将保存在M-K培养基中的供体角膜材料置于隔热环境中，如聚苯乙烯泡沫塑料容器中，并与温度低于0摄氏度的冰接触，它很容易结冰。为确保M-K培养基的推荐温度（+4摄氏度）得到妥善维持，所用的冰应在室温下放置，直到其表面呈现出闪亮、湿润的外观。如果使用这种闪亮湿润、正在融化的冰，已冷却至+4摄氏度的M-K培养基可以安全地保存在隔热良好的聚苯乙烯泡沫塑料容器中，并在+4摄氏度下维持约24小时。
