Anderson S A, Story P G
J Ophthalmic Nurs Technol. 1996 Jan-Feb;15(1):19-20.
It is important to evaluate donor corneas and media carefully prior to surgery. The eye banks that are responsible for preservation and transportation of donor corneal tissue should continue their efforts to prevent tissue damage, which includes careful placement of vials in properly prepared containers. If placed in direct contact with ice, the ice should be relatively warm and glistening. Direct contact with hard, super-cooled ice should be avoided. If living tissue is frozen without special treatment, ice crystals form in the cells and cell membrane rupture, causing death of the cell. Also, when ice crystals are present, tissue damage could take place when physical impact is caused by motion of the container during transportation.
在手术前仔细评估供体角膜和介质非常重要。负责供体角膜组织保存和运输的眼库应继续努力防止组织损伤，这包括将小瓶小心放置在妥善准备的容器中。如果直接与冰接触，冰应该相对温暖且有光泽。应避免与坚硬、过冷的冰直接接触。如果活体组织未经特殊处理就被冷冻，细胞内会形成冰晶，细胞膜破裂，导致细胞死亡。此外，当存在冰晶时，运输过程中容器的移动造成物理冲击时，可能会发生组织损伤。
