Petersen B J, Cornell N W, Veech R L
Adv Exp Med Biol. 1980;132:533-41. doi: 10.1007/978-1-4757-1419-7_55.
Studies of ethanol oxidation and other metabolic pathways in humans are often limited by the availability of a reproducible test material. Because of this we have tested human fibroblasts for ethanol metabolism and alcohol dehydrogenase content. Seven different cell lines have been studied and found to contain an enzymatic activity identified as alcohol dehydrogenase by the following criteria: it is NAD+-dependent, the Km for ethanol is like human liver, it is completely inhibited by 25 microM 4-pentylpyrazole. The fibroblast activity was analyzed by isoelectric focusing and found to contain several isozymes also present in the human liver sample. In addition, fibroblasts contain 2 major isozymes which migrate anodally to any isozymes previously reported in human liver. Thus, fibroblasts appear to be useful material for comparing enzymatic aspects of ethanol metabolism in alcoholics and nonalcoholics.
对人类乙醇氧化及其他代谢途径的研究常常受到可重复测试材料可用性的限制。因此，我们对人类成纤维细胞进行了乙醇代谢和乙醇脱氢酶含量的测试。我们研究了七种不同的细胞系，发现它们含有一种符合以下标准的酶活性，该活性被鉴定为乙醇脱氢酶：它依赖于NAD +，对乙醇的Km值与人类肝脏相似，它被25微摩尔4 - 戊基吡唑完全抑制。通过等电聚焦分析成纤维细胞活性，发现其含有几种也存在于人类肝脏样本中的同工酶。此外，成纤维细胞含有两种主要同工酶，它们向阳极迁移，超过了先前在人类肝脏中报道的任何同工酶。因此，成纤维细胞似乎是比较酗酒者和非酗酒者乙醇代谢酶学方面的有用材料。
