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不同生长条件对热解气-液相色谱法鉴别三种细菌的影响。

Effect of different growth conditions on the discrimination of three bacteria by pyrolysis gas-liquid chromatography.

作者信息

Gutteridge C S, Norris J R

出版信息

Appl Environ Microbiol. 1980 Sep;40(3):462-5. doi: 10.1128/aem.40.3.462-465.1980.

Abstract

High-resolution pyrolysis gas-liquid chromatography was applied to three bacteria (Escherichia coli NCTC 9001, Pseudomonas putida (NCIB 9494, and Staphylococcus aureus NCTC 8532) grown under a variety of conditions. Changing the culture medium drastically altered the quantitative aspects of the pyrograms of all three organisms, but the effects of culture time and incubation temperature were less severe. Mathematical analysis of the relative peak heights showed that four peaks could be used to discriminate the three bacteria however they were cultured.

摘要

高分辨率热解气相-液相色谱法应用于在多种条件下培养的三种细菌(大肠杆菌NCTC 9001、恶臭假单胞菌(NCIB 9494)和金黄色葡萄球菌NCTC 8532)。大幅改变培养基会显著改变所有三种微生物热解图的定量特征,但培养时间和培养温度的影响较小。对相对峰高的数学分析表明,四个峰可用于区分这三种细菌,无论它们如何培养。

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