Jacobs N J, Jacobs J M, Brent P
J Bacteriol. 1970 May;102(2):398-403. doi: 10.1128/jb.102.2.398-403.1970.
The enzyme system capable of converting coproporphyrinogen to protoporphyrin was demonstrated in the soluble fraction of extracts of Pseudomonas fluorescens grown aerobically, of P. denitrificans grown anaerobically under denitrifying conditions, and of Escherichia coli grown both aerobically and anaerobically. Protoporphyrin accumulation by each of these extracts occurred only if the assay was conducted aerobically. Attempts to replace this oxygen requirement with several alternate electron acceptors were not successful. The conversion of coproporphyrinogen to protoporphyrin could not be demonstrated in extracts of the heme-containing organisms Staphylococcus epidermidis and Bacillus subtilis.
在需氧生长的荧光假单胞菌提取物的可溶部分、在反硝化条件下厌氧生长的反硝化假单胞菌提取物的可溶部分以及需氧和厌氧生长的大肠杆菌提取物的可溶部分中,均证实了能够将粪卟啉原转化为原卟啉的酶系统。只有在需氧条件下进行测定时,这些提取物中的每一种才会积累原卟啉。尝试用几种替代电子受体来替代这种对氧气的需求均未成功。在含血红素的生物体表皮葡萄球菌和枯草芽孢杆菌的提取物中,无法证实粪卟啉原向原卟啉的转化。