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在-196摄氏度成功冷冻保存大鼠心脏的耳廓碎片。

Successful cryopreservation of auricle fragments from rat hearts at -196 degrees C.

作者信息

Matthes G, Hackensellner H A, Hartwig A

出版信息

Experientia. 1978 Aug 15;34(8):1024-5. doi: 10.1007/BF01915323.

DOI:10.1007/BF01915323
PMID:700012
Abstract

The influence of electrolyte composition and glucose concentration of a cryoprotective medium on the survival of auricle fragments from adult rat hearts after storage at -196 degrees C was investigated. Using a K+-, Mg++-, Ca++-rich solution with increased glucose concentration, a high rate of surviving fragments was found after cryopreservation.

摘要

研究了低温保护介质的电解质组成和葡萄糖浓度对成年大鼠心脏耳廓碎片在-196℃储存后存活情况的影响。使用葡萄糖浓度升高的富含K +、Mg ++、Ca ++的溶液，冷冻保存后发现存活碎片的比例很高。

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1
Successful cryopreservation of auricle fragments from rat hearts at -196 degrees C.在-196摄氏度成功冷冻保存大鼠心脏的耳廓碎片。
Experientia. 1978 Aug 15;34(8):1024-5. doi: 10.1007/BF01915323.
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本文引用的文献

1
Resumption of heart-beat in chick embryo frozen in liquid nitrogen.液氮冷冻的鸡胚心跳恢复
Biodynamica. 1950 May;7(126-128):1-5.
2
In vitro preservation of functioning rabbit hearts in a depolarized state at 4 degrees C.4℃下兔心脏在去极化状态下的体外功能保存
Cryobiology. 1969 May-Jun;5(6):403-9. doi: 10.1016/s0011-2240(69)80105-7.
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Reanimation of myocardial cells preserved in the frozen state.
Nature. 1969 Jun 21;222(5199):1174-5. doi: 10.1038/2221174a0.
4
Pulsatile activity of isolated heart muscle cells after freezing storage.冷冻保存后离体心肌细胞的搏动活动。
Cryobiology. 1968 Jan-Feb;4(4):200-3. doi: 10.1016/s0011-2240(68)80116-6.
5
Resumption of rhythmic pulsations in small pieces of frog hearts after freezing in liquid nitrogen.在液氮中冷冻后青蛙心脏小块组织中节律性搏动的恢复。
Biodynamica. 1968;10(210):211-7.
6
Ultrastructure-function correlative studies for cardiac cryopreservation. I. Hearts perfused with various concentrations of dimethyl sulfoxide (DMSO).
Cryobiology. 1971 Jun;8(3):280-9. doi: 10.1016/0011-2240(71)90051-4.
7
The role of glucose in the survival and 'recovery' of the anoxic isolated perfused rat heart.葡萄糖在缺氧离体灌注大鼠心脏存活及“恢复”中的作用。
Biochem J. 1972 Aug;128(5):1125-33. doi: 10.1042/bj1281125.
8
Preservation of structure and function of smooth muscle cooled to -79 degrees C in unfrozen aqueous media.在未冷冻的水性介质中冷却至-79摄氏度时平滑肌结构和功能的保存。
Nat New Biol. 1972 Mar 15;236(63):58-60. doi: 10.1038/newbio236058a0.