Yamamoto K, Kakinuma M, Kato K, Okuyama H, Azuma I
Immunology. 1980 Aug;40(4):557-64.
Intravenous administration of 6-O-mycoloyl-N-acetylmuramyl-L-alanyl-D-isoglutamine (mycol-MDP) together with a specific antigen, PPD, in a water-in-oil emulsion was found to produce lung granuloma and to provide a low but significant grade of protection in mice against tuberculous infection within 4 weeks. However, these products, when given in an oil-in-water emulsion did not produce granuloma. Mycol-MDP alone produced comparable lung granuloma in both C57Bl/6 mice, high responders to BCG cell walls (CW), and C3H/He mice, low responders, 1 week after the injection, and when challenged at this time by aerosol containing virulent bovine tubercle bacilli, they showed significantly increased resistance. The present results confirmed the close relationship between lung granuloma and protection against aerosol challenge with Ravenel and revealed that the extent of lung granuloma at the time of aerosol challenge is crucial for the development of protection in mice immunized with mycol-MDP plus PPD as it is in mice immunized with BCG CW. However, these findings are not always the case for lung granuloma induced with mycol-MDP alone.
发现在油包水乳剂中静脉注射6-O-霉菌酰-N-乙酰胞壁酰-L-丙氨酰-D-异谷氨酰胺（霉菌酰-MDP）与特定抗原结核菌素纯蛋白衍生物（PPD）一起，可在小鼠体内产生肺肉芽肿，并在4周内为小鼠提供低但显著程度的抗结核感染保护。然而，这些产物以水包油乳剂形式给药时不会产生肉芽肿。单独注射霉菌酰-MDP在对卡介苗细胞壁（CW）反应高的C57Bl/6小鼠和反应低的C3H/He小鼠中，在注射后1周均产生了相当的肺肉芽肿，并且此时用含有强毒牛结核杆菌的气雾剂进行攻击时，它们显示出显著增强的抵抗力。目前的结果证实了肺肉芽肿与抵抗拉夫内尔气雾剂攻击之间的密切关系，并表明在用霉菌酰-MDP加PPD免疫的小鼠中，气雾剂攻击时肺肉芽肿的程度对于保护作用的发展至关重要，就像在用卡介苗细胞壁免疫的小鼠中一样。然而，对于单独用霉菌酰-MDP诱导的肺肉芽肿，情况并非总是如此。
