Vriend R A, Geissinger H D
J Microsc. 1980 Sep;120(Pt 1):53-64. doi: 10.1111/j.1365-2818.1980.tb04119.x.
The procedure for direct intermicroscopic (LM leads to SEM leads to TEM) correlation of muscle was modified. The major modification was the use of cryostat sections which showed far better ultrastructural preservation than did paraffin sections. The use of an osmium-thiocarbohydrazide ligand binding procedure (OTOTO) did not noticeably improve the scanning electron microscopic (SEM) image, but did result in a subsequent transmission electron microscopic (TEM) image which showed increased contrast of the intracellular membranous components. Besides improving the preservation at the TEM level of examination, the techniques used to locate the same structure or area previously examined under the complementary modes of microscopy (i.e. LM and SEM), were also refined.
肌肉直接显微镜（光镜转扫描电镜再转透射电镜）关联程序被修改。主要修改之处在于使用了低温恒温器切片，其超微结构保存状况远优于石蜡切片。使用锇-硫代碳酰肼配体结合程序（OTOTO）并未显著改善扫描电子显微镜（SEM）图像，但确实使随后的透射电子显微镜（TEM）图像显示出细胞内膜性成分对比度增加。除了在透射电镜检查层面改善保存状况外，用于定位先前在互补显微镜模式（即光镜和扫描电镜）下检查过的相同结构或区域的技术也得到了改进。
