Ogata T, Yamasaki Y
Department of Surgery, Kochi Medical School, Japan.
Scanning Microsc. 1993 Mar;7(1):145-56.
The three-dimensional structure of the sarcoplasmic reticulum (SR), transverse (T)-axial tubular system and mitochondria in various muscles was examined by means of ultra-high resolution scanning electron microscopy (SEM) after removal of the cytoplasmic matrices and myofilaments by the aldehyde-osmium-DMSO-osmium procedure. The striated muscles reviewed and presented are twitch and slow fibers of the frog, twitch and slow fibers of the chicken, twitch extrafusal fibers and intrafusal fibers of the rat, and cardiac muscle fibers of the rat and dog. In all of these striated muscle fibers, T-tubules run transversely and are coupled with terminal cisternae forming triads or dyads. Sarcotubules arising from the terminal cisterna form meshes around the myofibrils. Considerable variations are seen in the location of the T-tubules, the structure of the terminal cisternae, the SR and the mitochondria among these muscles. The changes of these organelles in the experimental pathological conditions, (i.e., experimental mitochondrial myopathy and hypertrophic myocardium of spontaneously hypertensive rats) are also presented. In addition the SR and mitochondria in the smooth muscles of the rodents are described and discussed.
通过醛-锇-二甲基亚砜-锇法去除细胞质基质和肌丝后，利用超高分辨率扫描电子显微镜（SEM）对各种肌肉中肌浆网（SR）、横（T）轴管状系统和线粒体的三维结构进行了检查。所回顾和展示的横纹肌包括青蛙的快肌纤维和慢肌纤维、鸡的快肌纤维和慢肌纤维、大鼠的快肌梭外纤维和梭内纤维，以及大鼠和狗的心肌纤维。在所有这些横纹肌纤维中，T小管横向运行，并与终池相连形成三联体或二联体。从终池产生的肌小管在肌原纤维周围形成网。在这些肌肉中，T小管的位置、终池的结构、肌浆网和线粒体存在相当大的差异。还展示了这些细胞器在实验性病理条件下（即实验性线粒体肌病和自发性高血压大鼠的肥厚性心肌）的变化。此外，还对啮齿动物平滑肌中的肌浆网和线粒体进行了描述和讨论。
