Aymard M, Million J, Kessler N
Pathol Biol (Paris). 1980 Oct;28(8):535-9.
A modification of the single radial haemolysis test by treatment with Cl3Cr of the virus coated red blood cells is proposed for a rapid, more sensitive and reproducible serodiagnosis of Influenza infections. The reagents "red blood cells-virus-Cl3Cr" is stable at 4 degrees C for at least 1 month: the modified test gives results within 3 hours, in measuring the H-anti-H specific reactions. The anti-influenza A immune response was studied in patients of different age, bled at different time after exposure. The IgM do not diffuse in the agarose and do not haemolyse. A clear haemolytic area is produced by IgG, partially haemolysed areas occur when there is a competition between specific IgM and IgG and in the case of reactions involving cross-reacting antigenic determinants.
提出了一种通过用氯化铬(Cl3Cr)处理病毒包被的红细胞来改良单径向溶血试验的方法,用于流感感染的快速、更灵敏且可重复的血清学诊断。“红细胞-病毒-氯化铬”试剂在4℃下至少可稳定保存1个月:改良后的试验在测量H抗-H特异性反应时,3小时内即可得出结果。研究了不同年龄患者在接触病毒后不同时间采血时的抗甲型流感免疫反应。IgM不会在琼脂糖中扩散且不会溶血。IgG会产生清晰的溶血区,当特异性IgM和IgG之间存在竞争时,以及在涉及交叉反应抗原决定簇的反应情况下,会出现部分溶血区。