Bruenn J, Bobek L, Brennan V, Held W
Nucleic Acids Res. 1980 Jul 11;8(13):2985-97. doi: 10.1093/nar/8.13.2985.
ScV-L is a simple double-stranded RNA virus of yeast, consisting of a 4.8 kilobase pair double-stranded RNA (L) encapsidated in isometric particles composed mainly of one polypeptide (ScV-Pl) of 88,000 daltons. L encodes ScV-Pl. There is a capsid-associated RNA polymerase that synthesizes in vitro predominantly single-stranded RNA. We show that this polymerase activity is a transcriptase, at the least one product of which is the mRNA for ScV-Pl. The transcript, like its template, is uncapped.
ScV-L是一种简单的酵母双链RNA病毒,由4.8千碱基对的双链RNA(L)组成,该双链RNA被包裹在主要由一个88,000道尔顿的多肽(ScV-Pl)构成的等轴颗粒中。L编码ScV-Pl。有一种与衣壳相关的RNA聚合酶,其在体外主要合成单链RNA。我们发现这种聚合酶活性是一种转录酶,其至少一种产物是ScV-Pl的mRNA。该转录本与其模板一样,没有帽结构。