酵母双链RNA杀伤病毒基因的定位

Welsh J D, Leibowitz M J

Proc Natl Acad Sci U S A. 1982 Feb;79(3):786-9. doi: 10.1073/pnas.79.3.786.

The M double-stranded RNA (ds RNA) genome segment of the cytoplasmically inherited killer virus of yeast codes for two polypeptides when denatured and translated in vitro: a previously known 32,000-dalton peptide and a newly discovered 19,000-dalton peptide (NaDodSO4/polyacrylamide gel electrophoresis). An internal 190-base-pair region of the ds RNA is selectively degraded by S1 nuclease treatment at 65 degrees C, resulting in two ds RNA fragments which contain the termini of the original ds RNA. The larger fragment codes for the 32,000-dalton polypeptide and the smaller fragment codes for the 19,000-dalton polypeptide. Thus, the two gene products of M are encoded by distinct regions of this ds RNA.

酵母细胞质遗传杀伤病毒的M双链RNA（ds RNA）基因组片段在体外变性并翻译时编码两种多肽：一种是先前已知的32000道尔顿的肽，另一种是新发现的19000道尔顿的肽（十二烷基硫酸钠/聚丙烯酰胺凝胶电泳）。ds RNA的一个190个碱基对的内部区域在65℃下经S1核酸酶处理后被选择性降解，产生两个ds RNA片段，它们包含原始ds RNA的末端。较大的片段编码32000道尔顿的多肽，较小的片段编码19000道尔顿的多肽。因此，M的两种基因产物由该ds RNA的不同区域编码。