[大肠杆菌MRE 600合成赖氨酸脱羧酶的条件]
[Conditions of the synthesis of lysine decarboxylase by Escherichia coli MRE 600].
作者信息
Beretskene S Ia, Bruzgulis P A, Ragavichus A B
出版信息
Prikl Biokhim Mikrobiol. 1980 May-Jun;16(3):351-5.
PMID:7001435
Abstract
The effect of aeration, pH of the cultivation medium, temperature and time of cultivation of Escherichia coli MRE600 on the synthesis of cellular lysine decarboxylase (LDC) was studied. It was demonstrated that LDC reached maximum activity in the 5-hour bacterial culture cultivated in the nutrient medium with pH 6.0 at 29--30 degrees C. Aeration increased the yield by about 3 times and decreased LDC activity by 5.5 times.
摘要
研究了通气、培养基pH值、温度以及培养时间对大肠杆菌MRE600细胞赖氨酸脱羧酶（LDC）合成的影响。结果表明，在29 - 30摄氏度、pH值为6.0的营养培养基中培养5小时的细菌培养物中，LDC活性达到最大值。通气使产量提高了约3倍，但使LDC活性降低了5.5倍。
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