Sakai A, Yakushiji K, Mashimo S
Transplant Proc. 1980 Sep;12(3 Suppl 1):74-81.
The ability of isolated skin and kidney cells to activate allogeneic lymphocytes in vitro was studied in the MHC-identical and incompatible rat strains. The preparation of these cells and optimal culture conditions were summarized. 3H-thymidine uptake was strongest with skin epidermal cells and weakest with kidney cells. The results obtained from fractionated cells indicated that both Langerhans (Ia positive) and other cells (Ia negative) of the skin epidermis stimulated lymphocytes equally well and that only glomerulus and whole kidney cells stimulated, whereas cells fom the medulla and cultured epithelium failed to do so. It was suggested that skin epidermal cells possess lymphocyte-activating antigens other than Ia antigens that may be responsible for MHC-identical graft rejection. The weaker reaction by kidney cells and the limited localization of lymphocyte-activating determinants suggest that kidney cells may not express specific alloantigens detectable by the procedure employed; these cells obviously carry only a limited amount of Ia antigens.
在 MHC 相同和不相容的大鼠品系中，研究了分离的皮肤和肾细胞在体外激活同种异体淋巴细胞的能力。总结了这些细胞的制备方法和最佳培养条件。3H-胸腺嘧啶核苷摄取以皮肤表皮细胞最强，肾细胞最弱。从分离细胞获得的结果表明，皮肤表皮的朗格汉斯细胞（Ia 阳性）和其他细胞（Ia 阴性）刺激淋巴细胞的效果相同，只有肾小球和全肾细胞能刺激淋巴细胞，而来自髓质和培养上皮的细胞则不能。有人提出，皮肤表皮细胞除了 Ia 抗原外还拥有淋巴细胞激活抗原，这可能是 MHC 相同移植排斥反应的原因。肾细胞反应较弱以及淋巴细胞激活决定簇的有限定位表明，肾细胞可能不表达所用方法可检测到的特异性同种异体抗原；这些细胞显然仅携带有限量的 Ia 抗原。
