Schreiner G F, Kiely J M, Cotran R S, Unanue E R
J Clin Invest. 1981 Oct;68(4):920-31. doi: 10.1172/jci110347.
The existence of a subpopulation of rat glomerular cells bearing Ia determinants has been demonstrated with the aid of techniques for the enzymatic isolation and culture of glomerular cells. The Ia-positive cell is normally resident in the uninflamed glomerulus. It resembles a mononuclear phagocyte and consists of a functionally heterogeneous cell population with the capacity of Fc receptor display and phagocytosis, both in vivo and in vitro. A new technique for labeling these cells in situ in intact glomeruli has indicated that Ia-positive cells make up approximately 2% of the total glomerular cell population. The isolated glomerular cells can take up antigen and stimulate immune lymphocytes in an I-region-restricted interaction. They are strongly stimulatory in an allogeneic primary mixed lymphocyte culture. Characterization of this cell type suggests potential new insights into the pathogenesis of renal allograft rejection and immunologically mediated glomerulonephritis.
借助肾小球细胞的酶促分离和培养技术，已证实存在带有Ia决定簇的大鼠肾小球细胞亚群。Ia阳性细胞通常存在于未发炎的肾小球中。它类似于单核吞噬细胞，由功能异质性的细胞群体组成，在体内和体外均具有Fc受体展示和吞噬能力。一种在完整肾小球中原位标记这些细胞的新技术表明，Ia阳性细胞约占肾小球细胞总数的2%。分离出的肾小球细胞可以摄取抗原，并在I区限制的相互作用中刺激免疫淋巴细胞。它们在同种异体原发性混合淋巴细胞培养中具有强烈的刺激作用。对这种细胞类型的表征提示了对肾移植排斥反应和免疫介导的肾小球肾炎发病机制的潜在新见解。
