体外检测人多形核白细胞（PMNL）来源的溶酶体蛋白的杀菌特性。I. 溶酶体蛋白所得组分的特性

Bactericidal properties of lysosomal proteins obtained from human polymorphonuclear leucocytes (PMNL) examined in vitro. I. Characteristics of obtained fractions of lysosomal proteins.

作者信息

Kowalska M, Denys A

出版信息

Zentralbl Bakteriol Mikrobiol Hyg B. 1980 Jun;171(1):55-63.

Abstract

In the present investigation the fractions obtained from lysosomal proteins of human peripheral blood granulocytes were analysed morphologically. In the analysis a column with DEAE-cellulose and ultracentrifugation in stepwise saccharose were employed. Electro-microscopic examinations of every fraction of lysosomes pointed to their morphological differences; the highest contents of lysosomal bags was noted in fraction number III. Correlations between activity of "indicatory" enzymes and content of protein and bactericidal activity in four studied fractions were discovered.

摘要

在本研究中，对从人外周血粒细胞溶酶体蛋白中获得的各组分进行了形态学分析。分析过程中使用了装有二乙氨基乙基纤维素的柱以及在逐步蔗糖溶液中进行超速离心。对溶酶体的每个组分进行电子显微镜检查，结果显示它们在形态上存在差异；在组分III中溶酶体囊泡的含量最高。研究发现了四个研究组分中“指示性”酶的活性、蛋白质含量和杀菌活性之间的相关性。

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Bactericidal properties of lysosomal proteins obtained from human polymorphonuclear leucocytes (PMNL) examined in vitro. I. Characteristics of obtained fractions of lysosomal proteins.体外检测人多形核白细胞（PMNL）来源的溶酶体蛋白的杀菌特性。I. 溶酶体蛋白所得组分的特性

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