Gaszyński W, Pruszyńska-Bednarek A, Majka A, Siwik J
Anaesth Resusc Intensive Ther. 1976 Jul-Sep;4(3):187-94.
The effect of bone cement used for stabilization of hip joint endoprosthesis on the activity of lysosomal enzymes in peripheral blood granulocytes was studied. The operations were carried out under balanced, non-toxic, endotracheal anaesthesia using muscle relaxants. The blood for tests was obtained from an antecubital vein before and during anaesthesia, before application of bone cement, and 10 min., 1 hour and 24, 48 and 72 hours after endoprosthesis implantation using bone cement. The activity of acid phosphatase, acid protease and neutral protease was determined by calculating it in relation to protein level in the studied fractions. During anaesthesia as well as during the operation when bone cement was applied no statistically significant differences were found in the activity of lysosomal enzymes. On the other hand, after the operation statistically significant changes were found in the activity of lysosomal marker enzymes. Lability of lysosomal membranes appeared with permeation of hydrolases into supernatant. These results confirmed the importance of lysosomal proteins in the processes of wound healing and general postoperative course.
研究了用于髋关节假体固定的骨水泥对外周血粒细胞溶酶体酶活性的影响。手术在使用肌肉松弛剂的平衡、无毒气管内麻醉下进行。测试血液在麻醉前和麻醉期间、骨水泥应用前以及使用骨水泥植入假体后10分钟、1小时、24小时、48小时和72小时从肘前静脉采集。通过计算与所研究组分中蛋白质水平相关的值来测定酸性磷酸酶、酸性蛋白酶和中性蛋白酶的活性。在麻醉期间以及应用骨水泥的手术过程中，未发现溶酶体酶活性有统计学上的显著差异。另一方面，手术后溶酶体标记酶的活性出现了统计学上的显著变化。随着水解酶渗透到上清液中，溶酶体膜出现不稳定。这些结果证实了溶酶体蛋白在伤口愈合过程和术后总体病程中的重要性。
